Aim: To study pollen germination on a slide.
Apparatus and materials required: Fresh seasonal flower slides, microscope, beaker, cavity slide, boric acid, sucrose.
Procedure:
Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water.
Take a few drops of this solution on a clear slide, dust a few pollen grains from the stamen of a mature flower in it.
Obverse the slide under the microscope after 5 minutes. Keep observing the slide regularly after intervals of half an hour.
Observations:
In a nutrient solution or nutrient-rich medium, the pollen grains germinate. The tube/vegetative cell enlarges and comes out the pollen grains through one of the germ pores to form a pollen tube. The generative cell nucleus soon passes into it and dives to make 2 sperm nuclei (male gametes). Each male gamete is lenticular to spherical in outline.
Precautions:
Flowers should be freshly picked
Use a clear cavity slide to observe pollen grain
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Please post the clear pictures please
Very good
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Like
Not bad
Also give result and conclusion of the experiment
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NOT YET
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Conclusion?
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What is the principle of in vitro pollen germination …?
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Is the figure right???
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Please give the conclusion
Thanks
Plz give the clear pic
Send me plz clear picture
Why Boric acid is added?
Good
Thanks!! This is really very helpful 😊😊
THANKS !! THIS IS REALLY VERY HELPFUL 😊😊
Thanks!! This is really helpful 😇😇
This helped a lot thanks for posting