Oil Fat Test

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AIM: To test the presence of oil or fat in the given food sample.

 PROCEDURE:

S. No. Experiment Obervation Inference
1

SOLUBILITY TEST

Food sample + water

 Food sample + chloroform(CHCl3)

Does not dissolve Miscible Oil / fat present.
OR
2

SPOT TEST

Smear the food sample on paper.

A translucent spot is observed. Oil / fat present.
OR
3

ACROLEIN TEST

Food sample + KHSO4. Heat

An irritating odour is obtained. Oil / fat present.
Spot Test Showing Presence of Fat

EQUATIONS: (ON BLANK SIDE USING A PENCIL)

      Oil/ fat               –>                    glycerol + fatty acid

CH(OH)CH(OH) CH(OH)    +    KHSO4 –>     CH2═CHCHO(acrolein)   + 2H2O

RESULT:  (ON RULED SIDE )  The food sample has been tested for oil/fat

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