Viva Voce – Questions on Protein, Fat, Carbohydrate Test

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  • What are proteins? Name their basic unit.

Proteins are complex nitrogenous organic compounds of high molecular masses. Amino acids are the basic unit of proteins.

  • What is the ring due to in Molisch’s test?

Concentrated sulphuric acid (H2SO4) produces furfural or its derivative on reacting with carbohydrates.  This furfural forms a violet coloured compound on coupling with naphthol.

  • What is the chemistry of Acrolein Test?

Oil or fat gets hydrolyzed to free glycol which forms pungent smelling acrolein on further reaction.

Chemical Equation of Acrolein Test
Chemical Equation of Acrolein Test

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